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the hGCSF fusion protein at 30uC, whereas the solubilities of the Trx-, GST-, and His6-tagged proteins were low at this temperature. MBP is thought to act as a general molecular chaperone by binding to hydrophobic residues present on protein surfaces. MBP-tagged proteins can be easily purified with commercially available MBP-binding columns. PDI forms and breaks disulfide bonds of proteins in the
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