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redicted relatively low solubility for the MBP, Trx, and GST tags , despite the fact that hGCSF fused with these tags showed good solubility. With the exception of His6-hGCSF, lowering the expression temperature from 30uC to 18uC increased the solubility of all Purification step hGCSF purified from PDIb'a'-hGCSF Total protein Purity 69.1 73.3 99 30.8 16.7 11.3 hGCSF Yield 100 54 36.7 hGCSF purifie
as mutated to Ser demonstrated that Cys18 is not required for bioactivity of hGCSF. However, during folding of hGCSF, intermolecular disulfide Soluble Overexpression and Purification of hGCSF N bonds between two Cys18 residues or Cys18 and another Cys residue can occur in aggregates. The formation of subsequent dimers or multimers can render hGCSF insoluble in E. coli cytoplasm. As a result of the
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as mutated to Ser demonstrated that Cys18 is not required for bioactivity of hGCSF. However, during folding of hGCSF, intermolecular disulfide Soluble Overexpression and Purification of hGCSF N bonds between two Cys18 residues or Cys18 and another Cys residue can occur in aggregates. The formation of subsequent dimers or multimers can render hGCSF insoluble in E. coli cytoplasm. As a result of the
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